Cell Transplantation Kinetics
You are given a dataset for blood platelet levels after transplantation of megakaryocytes (MK) for both intravenous and intra-bone routes. Assuming that 100% of fragmented platelets are derived from 100% transplanted MKs, determine MK decay kinetics:
i) Plot % MK decay (linear) vs Time (linear) and determine an appropriate curve fit – provide an equation with numbers. Note that you will have to do conversion from the given raw data. Determine half-life for each curve.
ii) Describe what this data tells us about differences between intravenous and intra-bone transplantation of MKs to generate platelets. Describe mechanisms behind how platelets may be generated from MKs via each route of transplantation, based on this data.
| Time (hr) | Intrabone |
| 1 | 6.58 |
| 8 | 15.49 |
| 24 | 15.49 |
| 48 | 31.37 |
| 72 | 24.78 |
| 168 | 0.64 |
| 336 | 4.20 |
| Time (hr) | Intravenous |
| 0.5 | 14.81 |
| 3 | 37.04 |
| 9 | 22.22 |
| 24 | 18.52 |
| 47 | 7.41 |
